Male and Female Genetic Linkage Maps of Fugu

The tiger pufferfish, Takifugu rubripes (fugu) was proposed as a genomic model because of its compact genome. The genome of fugu was sequenced to the draft level in 2002 as the second vertebrate genome to be fully sequenced. However, the assembly of the draft genome sequence is highly fragmented due to the lack of a genetic or a physical map. To determine the long-range linkage relationship of the sequences, we have constructed the first genetic linkage map for fugu (Kai et al. 2005). The maps for the male and female spanning 697.1 and 1,213.5 cM, respectively, were arranged into 22 linkage groups. The consolidated map consists of 200 microsatellite loci physically linked to genome sequences spanning ~40 Mb in total and contains ~4,000 gene models. This map provides a foundation for development of a complete physical map, a basis for comparison of long-range linkage of genes with other vertebrates, and a resource for mapping loci responsible for phenotypic diversity among Takifugu species.

Click each LG for details.

Scaffold numbers are on the basis of fugu v.3. Updating the map data from v3 to v4 is in progress.

Wataru Kai, Kiyoshi Kikuchi, Masashi Fujita, Hiroaki Suetake, Atushi Fujiwara, Yasutoshi Yoshiura, Mitsuru Ototake, Byrappa Venkatesh, Kadoo Miyaki, and Yuzuru Suzuki

A genetic linkage map for the tiger pufferfish, Takifugu rubripes.

Genetics, 171, 227-238 (2005)

For questions or comments, please contact Dr. Kikuchi.

Fisheries Laboratory

Graduate School of Agricultural and Life Sciences

The University of Tokyo